The original cryoprotecting solution for freezing leukocytes at low temperature (–80℃) has been created in the Institute of Physiology jointly with the Kirov Research Institute of Hematology and Blood Transfusion. The solution contains exo- and endocellular cryoprotector hexamethylenebistetrahydr oxyethylurea, endocellular cryoprotector dimethylsulfoxide, an antioxidant hydroxymethylethylpyridine succinate, and water for injections. The solution ensures long-term storage of leukocytes in the physiologically active condition.

An important distinctive feature is the presence of protectors of different types of action in the cryoprotector solute composition, which stabilize intracellular and extracellular water. That ensures more effective long-term preservation of leukocytes as compared with the currently used monoprotector solutions. All components of a solution are made in Russia and have low cost. According to biological tests, this solution is harmlessness for an organism that allows to use it in establishments of a biological and medical structure.